Conditioned medium-preconditioned EPCs enhanced the ability in oligovascular repair in cerebral ischemia neonatal rats
نویسندگان
چکیده
Abstract Background Oligovascular niche mediates interactions between cerebral endothelial cells and oligodendrocyte precursor (OPCs). Disruption of OPC-endothelium trophic coupling may aggravate the progress white matter injury (WMI) because could not provide sufficient support under diseased conditions. Endothelial progenitor (EPCs) have been reported to ameliorate WMI in adult brain by boosting oligovascular remodeling. It is necessary clarify role conditioned medium from hypoxic preconditioned EPCs (EC-pEPCs) since usually were recruited play important roles blood-brain barrier disruption. Here, we investigated effects EC-pEPCs on remodeling a neonatal rat model WMI. Methods In vitro, OPC apoptosis induced oxygen-glucose deprivation-injured microvascular (OGD-EC-CM) was analyzed TUNEL FACS. The EC damage expression cytomokine C-X-C motif ligand 12 (CXCL12) examined western blot effect CM against also verified silencing RNA. vivo, P3 pups subjected right common carotid artery ligation hypoxia treated with or at P7, then angiogenesis myelination together cognitive outcome evaluated 6th week. Results enhanced function decreased apoptosis. Meanwhile, it confirmed that OGD-EC-CM an increase CXCL12 EPCs, CXCL12-CXCR4 axis key signaling CXCR4 knockdown alleviated anti-apoptosis OPCs. number protein level markedly increased rats. Compared significantly apoptosis, vascular density corpus callosum, improved learning memory deficits model. Conclusions more effectively promote via
منابع مشابه
Cerebral Ischemia-Reperfusion Injuries in Vanadyl-Treated Diabetic Rats
Background: Ischemic stroke recovery is poor in diabetic mellitus (DM). Vanadium compounds (vanadium) relieve DM signs, but their influences on cerebral ischemia/reperfusion injury (I/RI) are inconclusive. Herein, the intensity of I/RI was inspected in vanadium-treated DM rats.Methods: Rats made diabetic with a single intravenous dose of streptozocin (39 mg/kg). Normal and DM rats used water or...
متن کاملCerebral Ischemia in Rats
Background and Purpose: Recent studies have suggested that cerebral infarction influences autonomic activity and may contribute to sudden death. The goal of this study was to examine effects of focal cerebral infarction on mean arterial pressure and heart rate. Methods: Halothane-anesthetized rats were assigned to two groups: stroke (n=10), in which the middle cerebral artery or an adjacent ves...
متن کاملHypoxic-Preconditioned Bone Marrow Stem Cell Medium Significantly Improves Outcome After Retinal Ischemia in Rats
PURPOSE We have previously demonstrated the protective effect of bone marrow stem cell (BMSC)-conditioned medium in retinal ischemic injury. We hypothesized here that hypoxic preconditioning of stem cells significantly enhances the neuroprotective effect of the conditioned medium and thereby augments the protective effect in ischemic retina. METHODS Rats were subjected to retinal ischemia by ...
متن کاملthe effect of mesenchymal stem cell conditioned media on in vitro model of cerebral ischemia
متن کامل
the relationship between learners critical thinking ability and their performance in the reading sections of the tofel and ielts test
the study reflected in this thesis aims at finding out relationships between critical thinking (ct), and the reading sections of tofel and ielts tests. the study tries to find any relationships between the ct ability of students and their performance on reading tests of tofel and academic ielts. however, no research has ever been conducted to investigate the relationship between ct and the read...
15 صفحه اولذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Stem Cell Research & Therapy
سال: 2021
ISSN: ['1757-6512']
DOI: https://doi.org/10.1186/s13287-021-02157-4